Hotspot

atpB-rbcL

LSC · 59843-60612 · 770 bp

Back to Aglaonema

Pi 0.00587

MarkerSeek score 65.00

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 770
variable_sites: 4
pi: 0.00587
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTGAGCAATACGCCCTAGG	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.7	58.0	810-895 bp	1.0	73.6
atpB-rbcL_p2	GAGCAATACGCCCTAGGTTT	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.7	58.0	807-892 bp	1.0	73.6
atpB-rbcL_p3	TTTGAGCAATACGCCCTAGG	ACCAGCTTTGAATCCAACACT	50.0	42.9	57.7	58.1	809-894 bp	1.0	73.4
atpB-rbcL_p4	GAGCAATACGCCCTAGGTTT	ACCAGCTTTGAATCCAACACT	50.0	42.9	57.7	58.1	806-891 bp	1.0	73.4
atpB-rbcL_p5	GGGAAAAGCGACATCCAGTA	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.6	58.0	842-927 bp	1.0	73.1