Hotspot

atpH-atpI

LSC · 16399-17271 · 873 bp

Back to Aglaonema

Pi 0.0218

MarkerSeek score 71.50

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 873
variable_sites: 19
pi: 0.0218
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	TCACAAGCGGTATTCAAGCT	45.0	45.0	58.1	57.5	983-994 bp	1.0	77.1
atpH-atpI_p2	AACAGAAGCGGCAGAAATCA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.1	58.5	959-970 bp	1.0	76.8
atpH-atpI_p3	CAGCAGCAATAACAGAAGCG	TCACAAGCGGTATTCAAGCT	50.0	45.0	57.8	57.5	993-1004 bp	1.0	76.5
atpH-atpI_p4	GCCAATCCAGCAGCAATAAC	TCACAAGCGGTATTCAAGCT	50.0	45.0	57.8	57.5	1000-1011 bp	1.0	76.2
atpH-atpI_p5	CAGCAGCAATAACAGAAGCG	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.8	58.5	969-980 bp	1.0	76.2