Hotspot

trnS-GCU-trnG-UCC

LSC · 9803-11482 · 1680 bp

Back to Aglaonema

Pi 0.0173

MarkerSeek score 63.10

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1680
variable_sites: 28
pi: 0.0173
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	AGGACGAATCACACTTTTACCA	45.0	40.9	58.0	57.7	1723-1757 bp	1.0	65.2
trnS-GCU-trnG-UCC_p2	CGCACAACGGATTAGCAATC	AGGACGAATCACACTTTTACCA	50.0	40.9	57.9	57.7	1733-1767 bp	1.0	64.8
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	GGACGAATCACACTTTTACCAC	45.0	45.5	58.0	57.6	1722-1756 bp	1.0	64.8
trnS-GCU-trnG-UCC_p4	CGCACAACGGATTAGCAATC	GGACGAATCACACTTTTACCAC	50.0	45.5	57.9	57.6	1732-1766 bp	1.0	64.4
trnS-GCU-trnG-UCC_p5	TCGCACAACGGATTAGCAAT	AGGACGAATCACACTTTTACCA	45.0	40.9	58.3	57.7	1734-1768 bp	1.0	63.4