Hotspot

rpoC1

LSC · 15572-18354 · 2783 bp

Back to Ageratum

Pi 0.00639

MarkerSeek score 46.50

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2783
variable_sites: 13
pi: 0.00639
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TTCGGTTGTTCGTTCGAGAA	AGAATTACAACAGCCCTGCC	45.0	50.0	57.8	58.2	2903-2907 bp	1.0	81.4
rpoC1_p2	TTTCGGTTGTTCGTTCGAGA	AGAATTACAACAGCCCTGCC	45.0	50.0	57.8	58.2	2904-2908 bp	1.0	81.4
rpoC1_p3	TTCGGTTGTTCGTTCGAGAA	ATTCCCGCTGGTAGCATAGA	45.0	50.0	57.8	58.3	2920-2924 bp	1.0	80.4
rpoC1_p4	TTCGGTTGTTCGTTCGAGAA	TCCCGCTGGTAGCATAGAAT	45.0	50.0	57.8	58.3	2918-2922 bp	1.0	80.4
rpoC1_p5	TTTCGGTTGTTCGTTCGAGA	TCCCGCTGGTAGCATAGAAT	45.0	50.0	57.8	58.3	2919-2923 bp	1.0	80.4