Hotspot

rpoA

LSC · 76976-77905 · 930 bp

Back to Ageratum

Pi 0.0043

MarkerSeek score 61.20

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 930
variable_sites: 4
pi: 0.0043
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	TGTTTGACATCTTCTATGCGA	CATAATGGCTGTAGACCCCC	38.1	55.0	55.4	57.4	1069 bp	1.0	46.6
rpoA_p2	TGTTTGACATCTTCTATGCGAA	CATAATGGCTGTAGACCCCC	36.4	55.0	56.1	57.4	1069 bp	1.0	44.7
rpoA_p3	TTGTTTGACATCTTCTATGCGA	CATAATGGCTGTAGACCCCC	36.4	55.0	56.1	57.4	1070 bp	1.0	44.7
rpoA_p4	TGTTTGACATCTTCTATGCGA	ATAATGGCTGTAGACCCCCG	38.1	55.0	55.4	58.9	1068 bp	1.0	43.6
rpoA_p5	TGTTTGACATCTTCTATGCGAAA	CATAATGGCTGTAGACCCCC	34.8	55.0	56.8	57.4	1069 bp	1.0	42.2