Hotspot

petG

LSC · 65249-65353 · 105 bp

Back to Ageratum

Pi 0.0673

MarkerSeek score 84.30

Primer available yes

Label petG

Feature Metadata

label_name: petG
feature_type: gene
region: LSC
length_bp: 105
variable_sites: 7
pi: 0.0673
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG_p1	TCTTGGTCATTGAGATTCATGGT	GGAACTGGACCAGCAATCTT	39.1	50.0	57.8	57.8	280-281 bp	1.0	55.2
petG_p2	TCTTGGTCATTGAGATTCATGGT	CTGGAACTGGACCAGCAATC	39.1	55.0	57.8	58.5	282-283 bp	1.0	52.5
petG_p3	CTTGGTCATTGAGATTCATGGT	GGAACTGGACCAGCAATCTT	40.9	50.0	56.4	57.8	279-280 bp	1.0	51.8
petG_p4	TCTTGGTCATTGAGATTCATGGT	CCTACAGAGCGTGATTTGGA	39.1	50.0	57.8	57.3	334-335 bp	1.0	51.1
petG_p5	TCTTGGTCATTGAGATTCATGGT	TCCTACAGAGCGTGATTTGG	39.1	50.0	57.8	57.3	335-336 bp	1.0	51.1