Hotspot

rpl32-ndhF

SSC · 123083-124098 · 1016 bp

Back to Ageratum

Pi 0.00591

MarkerSeek score 62.20

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1016
variable_sites: 6
pi: 0.00591
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	CGCCCCATATCCTTTCCTTT	GTTGCCGTCGGAAAAAGAAG	50.0	50.0	57.6	58.0	1195-1217 bp	1.0	77.3
rpl32-ndhF_p2	CGCCCCATATCCTTTCCTTT	TGCCGTCGGAAAAAGAAGAA	50.0	45.0	57.6	58.0	1193-1215 bp	1.0	77.2
rpl32-ndhF_p3	CGCCCCATATCCTTTCCTTT	TTGCCGTCGGAAAAAGAAGA	50.0	45.0	57.6	58.0	1194-1216 bp	1.0	77.2
rpl32-ndhF_p4	TGACAAAGCTTTTAACGCCG	GTTGCCGTCGGAAAAAGAAG	45.0	50.0	57.6	58.0	1213-1235 bp	1.0	77.1
rpl32-ndhF_p5	TGACAAAGCTTTTAACGCCG	TGCCGTCGGAAAAAGAAGAA	45.0	45.0	57.6	58.0	1211-1233 bp	1.0	77.0