Hotspot

trnR-UCU-trnT-GGU

LSC · 29472-30626 · 1155 bp

Back to Ageratum

Pi 0.00346
MarkerSeek score 60.70
Primer available yes
Label trnR-UCU-trnT-GGU

Feature Metadata

label_name
trnR-UCU-trnT-GGU
feature_type
igs
region
LSC
length_bp
1155
variable_sites
4
pi
0.00346
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-trnT-GGU_p1 AGAAGACCTCTGTCCTATCCA ACGCGTTACCATGGGATTAC 47.6 50.0 56.9 58.1 1229 bp 1.0 63.4
trnR-UCU-trnT-GGU_p2 AGGTTTAGAAGACCTCTGTCCT ACGCGTTACCATGGGATTAC 45.5 50.0 57.9 58.1 1235 bp 1.0 62.7
trnR-UCU-trnT-GGU_p3 AGAAGACCTCTGTCCTATCCA CGCGTTACCATGGGATTACT 47.6 50.0 56.9 57.8 1228 bp 1.0 62.0
trnR-UCU-trnT-GGU_p4 AGAAGACCTCTGTCCTATCCA CTTACGCGTTACCATGGGAT 47.6 50.0 56.9 57.8 1232 bp 1.0 62.0
trnR-UCU-trnT-GGU_p5 AGGTTTAGAAGACCTCTGTCCT CGCGTTACCATGGGATTACT 45.5 50.0 57.9 57.8 1234 bp 1.0 61.3