Hotspot

trnC-GCA-petN

LSC · 8999-9656 · 658 bp

Back to Ageratum

Pi 0.00608

MarkerSeek score 61.70

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 658
variable_sites: 4
pi: 0.00608
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	AAAAGGGGATGCAAGTACGA	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.1	58.2	774-787 bp	1.0	72.2
trnC-GCA-petN_p2	CAAAAGGGGATGCAAGTACG	AGCCCAAGCGAGACTTACTA	50.0	50.0	57.1	58.2	775-788 bp	1.0	71.8
trnC-GCA-petN_p3	AGGATTCAAAAGGGGATGCA	AGCCCAAGCGAGACTTACTA	45.0	50.0	57.1	58.2	781-794 bp	1.0	71.8
trnC-GCA-petN_p4	AAAGGGGATGCAAGTACGAT	AGCCCAAGCGAGACTTACTA	45.0	50.0	56.9	58.2	773-786 bp	1.0	70.4
trnC-GCA-petN_p5	AGGATTCAAAAGGGGATGCAA	AGCCCAAGCGAGACTTACTA	42.9	50.0	57.8	58.2	781-794 bp	1.0	69.7