Hotspot

ndhE-psaC

SSC · 119265-119448 · 184 bp

Back to Ageratina

Pi 0.161

MarkerSeek score 85.90

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: SSC
length_bp: 184
variable_sites: 28
pi: 0.161
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 85.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	CTGAAGCCGCTATTGGACTC	ACATCCGTCCCAAGGTATCA	55.0	50.0	58.4	58.1	378-428 bp	1.0	83.3
ndhE-psaC_p2	CCGCTATTGGACTCGCTATT	ACATCCGTCCCAAGGTATCA	50.0	50.0	57.6	58.1	372-422 bp	1.0	83.3
ndhE-psaC_p3	CTGAAGCCGCTATTGGACTC	GGACACATTGAGTACACCCG	55.0	55.0	58.4	58.3	331-381 bp	1.0	83.2
ndhE-psaC_p4	CTGAAGCCGCTATTGGACTC	TTTGGCTTTACATCCGTCCC	55.0	50.0	58.4	58.2	387-437 bp	1.0	82.8
ndhE-psaC_p5	CCGCTATTGGACTCGCTATT	TTTGGCTTTACATCCGTCCC	50.0	50.0	57.6	58.2	381-431 bp	1.0	82.8