Hotspot

ycf15-trnV-GAC

IRb · 99984-100278 · 295 bp

Back to Ageratina

Pi 0.516

MarkerSeek score 72.10

Primer available yes

Label ycf15-trnV-GAC

Feature Metadata

label_name: ycf15-trnV-GAC
feature_type: igs
region: IRb
length_bp: 295
variable_sites: 116
pi: 0.516
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.76
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf15-trnV-GAC_p1	CCTTGCTTCTCTCACGGTAC	CCTCAGCCTACGGGGTATTA	55.0	55.0	58.0	58.0	891 bp	0.5	59.9
ycf15-trnV-GAC_p2	CCTTGCTTCTCTCACGGTAC	CATTGCGGAAAATTCCCCAC	55.0	50.0	58.0	58.0	1065 bp	0.5	59.9
ycf15-trnV-GAC_p3	GAAAGGACTCACTAAGCCGG	CCTCAGCCTACGGGGTATTA	55.0	55.0	58.0	58.0	790 bp	0.5	59.9
ycf15-trnV-GAC_p4	GAAAGGACTCACTAAGCCGG	CATTGCGGAAAATTCCCCAC	55.0	50.0	58.0	58.0	964 bp	0.5	59.8
ycf15-trnV-GAC_p5	TGACCTCCTTGCTTCTCTCA	CCTCAGCCTACGGGGTATTA	50.0	55.0	58.0	58.0	897 bp	0.5	59.8