Hotspot

atpB-rbcL

LSC · 53794-54797 · 1004 bp

Back to Ageratina

Pi 0.234

MarkerSeek score 92.70

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 1004
variable_sites: 174
pi: 0.234
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.74
markerseek_score: 92.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	922-1165 bp	1.0	83.6
atpB-rbcL_p2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	921-1164 bp	1.0	83.4
atpB-rbcL_p3	GGCAACCCCAGAACTAGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	57.9	58.0	849-1092 bp	1.0	82.9
atpB-rbcL_p4	GATACGCCCCAGGTTCTTTT	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.9	58.0	879-1122 bp	1.0	82.9
atpB-rbcL_p5	GGCAACCCCAGAACTAGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	57.9	58.1	848-1091 bp	1.0	82.7