Hotspot

psbM-trnD-GUC

LSC · 10863-11496 · 634 bp

Back to Ageratina

Pi 0.127

MarkerSeek score 96.80

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 634
variable_sites: 78
pi: 0.127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 96.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.6	58.3	751-768 bp	1.0	60.5
psbM-trnD-GUC_p2	AAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	40.0	50.0	54.5	58.3	745-762 bp	1.0	60.1
psbM-trnD-GUC_p3	ACTAAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.4	58.3	748-765 bp	1.0	59.1
psbM-trnD-GUC_p4	AGGAACTAAAATGAACAGTGCAG	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.3	58.3	752-769 bp	1.0	58.4
psbM-trnD-GUC_p5	AGGAACTAAAATGAACAGTGCA	TTCAATAGGCAAGAGCACCG	36.4	50.0	56.2	58.3	752-769 bp	1.0	57.6