Hotspot

trnC-GCA-petN

LSC · 9401-10199 · 799 bp

Back to Ageratina

Pi 0.104

MarkerSeek score 84.80

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 799
variable_sites: 76
pi: 0.104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	50.0	50.0	58.1	58.2	854-873 bp	1.0	89.2
trnC-GCA-petN_p2	TTTCCCCAGTTCAAATCCGG	CTAAAGCCCACTTCTTCCCC	50.0	55.0	58.1	57.9	917-936 bp	1.0	88.9
trnC-GCA-petN_p3	TTTCCCCAGTTCAAATCCGG	AAGCCCACTTCTTCCCCATA	50.0	50.0	58.1	58.3	914-933 bp	1.0	87.4
trnC-GCA-petN_p4	TTTCCCCAGTTCAAATCCGG	TAAAGCCCACTTCTTCCCCA	50.0	50.0	58.1	58.5	916-935 bp	1.0	85.5
trnC-GCA-petN_p5	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	50.0	50.0	58.1	57.1	853-872 bp	1.0	83.2