primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnC-GCA_p1	trnS-GCU-trnC-GCA	1	ACGGAAAGAGAGGGATTCGA	TCAGAGATTCTCGCGAGTCT	20	20	50.0	50.0	58.2	58.0	0.0	33.3	0.182607	8675	9328	1200-1253	1.000000	90.5
trnS-GCU-trnC-GCA_p2	trnS-GCU-trnC-GCA	2	AAATTAGGCTCGCCCCATTT	TCAGAGATTCTCGCGAGTCT	20	20	45.0	50.0	57.8	58.0	3.9	33.3	0.190438	8675	9328	1755-1808	1.000000	89.1
trnS-GCU-trnC-GCA_p3	trnS-GCU-trnC-GCA	3	ACGGAAAGAGAGGGATTCGA	AGTGAGGGGAAGCTTTTTGG	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.152424	8675	9328	988-988	0.500000	58.8
trnS-GCU-trnC-GCA_p4	trnS-GCU-trnC-GCA	4	AAATTAGGCTCGCCCCATTT	AGTGAGGGGAAGCTTTTTGG	20	20	45.0	50.0	57.8	58.0	3.9	0.0	0.160255	8675	9328	1543-1543	0.500000	58.7
trnS-GCU-trnC-GCA_p5	trnS-GCU-trnC-GCA	5	ACGGAAAGAGAGGGATTCGA	TAGTAATCCAAAGGCGGCAC	20	20	50.0	50.0	58.2	58.0	0.0	13.1	0.184029	8675	9328	827-827	0.500000	58.5
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	9401	10199	854-873	1.000000	89.2
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	CTAAAGCCCACTTCTTCCCC	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.212400	9401	10199	917-936	1.000000	88.9
trnC-GCA-petN_p3	trnC-GCA-petN	3	TTTCCCCAGTTCAAATCCGG	AAGCCCACTTCTTCCCCATA	20	20	50.0	50.0	58.1	58.3	0.0	0.0	0.400899	9401	10199	914-933	1.000000	87.4
trnC-GCA-petN_p4	trnC-GCA-petN	4	TTTCCCCAGTTCAAATCCGG	TAAAGCCCACTTCTTCCCCA	20	20	50.0	50.0	58.1	58.5	0.0	0.0	0.630679	9401	10199	916-935	1.000000	85.5
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	9401	10199	853-872	1.000000	83.2
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	23	20	39.1	50.0	57.6	58.3	0.0	0.0	3.700923	10863	11496	751-768	1.000000	60.5
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	20	20	40.0	50.0	54.5	58.3	0.0	0.0	3.756773	10863	11496	745-762	1.000000	60.1
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	ACTAAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	23	20	39.1	50.0	57.4	58.3	0.0	0.0	3.875520	10863	11496	748-765	1.000000	59.1
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	AGGAACTAAAATGAACAGTGCAG	TTCAATAGGCAAGAGCACCG	23	20	39.1	50.0	57.3	58.3	0.0	0.0	3.958013	10863	11496	752-769	1.000000	58.4
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	AGGAACTAAAATGAACAGTGCA	TTCAATAGGCAAGAGCACCG	22	20	36.4	50.0	56.2	58.3	0.0	0.0	4.069095	10863	11496	752-769	1.000000	57.6
trnY-GUA-rpoB_p1	trnY-GUA-rpoB	1	GGCGGGAGAAAAGATGGAAT	TCTGCCAAACCCTGATCAAC	20	20	50.0	50.0	57.9	58.1	0.0	0.0	0.148893	11768	12836	1606-1701	1.000000	90.5
trnY-GUA-rpoB_p2	trnY-GUA-rpoB	2	GGCGGGAGAAAAGATGGAAT	ACACGAACCCATATCCTTGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.096980	11768	12836	2029-2124	1.000000	88.6
trnY-GUA-rpoB_p3	trnY-GUA-rpoB	3	ACCCGTCCGAACTACAAAAC	ACACGAACCCATATCCTTGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.168613	11768	12836	2152-2247	1.000000	87.8
trnY-GUA-rpoB_p4	trnY-GUA-rpoB	4	CACCCGTCCGAACTACAAAA	ACACGAACCCATATCCTTGC	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.169918	11768	12836	2153-2248	1.000000	87.8
trnY-GUA-rpoB_p5	trnY-GUA-rpoB	5	GGATTCCCCGACCAATCAAA	ACACGAACCCATATCCTTGC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.176197	11768	12836	2271-2366	1.000000	87.4
atpB-rbcL_p1	atpB-rbcL	1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	58.0	58.0	0.0	0.0	1.047103	53794	54797	922-1165	1.000000	83.6
atpB-rbcL_p2	atpB-rbcL	2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	20	21	50.0	42.9	58.0	58.1	0.0	0.0	1.080391	53794	54797	921-1164	1.000000	83.4
atpB-rbcL_p3	atpB-rbcL	3	GGCAACCCCAGAACTAGAAG	CACCAGCTTTGAATCCAACAC	20	21	55.0	47.6	57.9	58.0	0.0	0.0	1.132608	53794	54797	849-1092	1.000000	82.9
atpB-rbcL_p4	atpB-rbcL	4	GATACGCCCCAGGTTCTTTT	CACCAGCTTTGAATCCAACAC	20	21	50.0	47.6	57.9	58.0	0.0	0.0	1.141542	53794	54797	879-1122	1.000000	82.9
atpB-rbcL_p5	atpB-rbcL	5	GGCAACCCCAGAACTAGAAG	ACCAGCTTTGAATCCAACACT	20	21	55.0	42.9	57.9	58.1	0.0	0.0	1.165895	53794	54797	848-1091	1.000000	82.7
rpl2_p1	rpl2	1	TGTAAAGACGAAGAAAGAAATTC	ACTTAATAGCATGGCGATACA	23	21	30.4	38.1	53.4	54.9	0.0	0.0	11.742856	84970	86250	1339-1534	1.000000	50.3
rpl2_p2	rpl2	2	TTTGTAAAGACGAAGAAAGAAA	ACTTAATAGCATGGCGATACA	22	21	27.3	38.1	52.2	54.9	0.0	0.0	11.859434	84970	86250	1341-1536	1.000000	50.3
rpl2_p3	rpl2	3	TTTTGTAAAGACGAAGAAAGAA	ACTTAATAGCATGGCGATACA	22	21	27.3	38.1	52.2	54.9	0.0	0.0	11.859434	84970	86250	1342-1537	1.000000	50.3
rpl2_p4	rpl2	4	TTTTTGTAAAGACGAAGAAAGA	ACTTAATAGCATGGCGATACA	22	21	27.3	38.1	52.2	54.9	0.0	0.0	11.859434	84970	86250	1343-1538	1.000000	50.3
rpl2_p5	rpl2	5	TTGTAAAGACGAAGAAAGAAATTC	ACTTAATAGCATGGCGATACA	24	21	29.2	38.1	54.1	54.9	0.0	0.0	11.968119	84970	86250	1340-1535	1.000000	50.3
rps12-ycf15_p1	rps12-ycf15	1	TACTCCGACAGCATCTAGGG	GTACCGTGAGAGAAGCAAGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.019671	98709	99791	1363-1363	0.500000	59.8
rps12-ycf15_p2	rps12-ycf15	2	AGCGATTCACATGGCATCAT	GTACCGTGAGAGAAGCAAGG	20	20	45.0	55.0	58.0	58.0	0.0	0.0	0.025275	98709	99791	1445-1445	0.500000	59.8
rps12-ycf15_p3	rps12-ycf15	3	TACTCCGACAGCATCTAGGG	GAATAGAACACGCTGAGCCA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.027485	98709	99791	1754-1754	0.500000	59.8
rps12-ycf15_p4	rps12-ycf15	4	TACTCCGACAGCATCTAGGG	CCGGCTTAGTGAGTCCTTTC	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.028880	98709	99791	1464-1464	0.500000	59.8
rps12-ycf15_p5	rps12-ycf15	5	TCTACAACGCACTAGAACGC	GTACCGTGAGAGAAGCAAGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.029193	98709	99791	1400-1400	0.500000	59.8
ycf15-trnV-GAC_p1	ycf15-trnV-GAC	1	CCTTGCTTCTCTCACGGTAC	CCTCAGCCTACGGGGTATTA	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.007701	99984	100278	891-891	0.500000	59.9
ycf15-trnV-GAC_p2	ycf15-trnV-GAC	2	CCTTGCTTCTCTCACGGTAC	CATTGCGGAAAATTCCCCAC	20	20	55.0	50.0	58.0	58.0	0.0	6.6	0.011083	99984	100278	1065-1065	0.500000	59.9
ycf15-trnV-GAC_p3	ycf15-trnV-GAC	3	GAAAGGACTCACTAAGCCGG	CCTCAGCCTACGGGGTATTA	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.016910	99984	100278	790-790	0.500000	59.9
ycf15-trnV-GAC_p4	ycf15-trnV-GAC	4	GAAAGGACTCACTAAGCCGG	CATTGCGGAAAATTCCCCAC	20	20	55.0	50.0	58.0	58.0	0.0	6.6	0.020292	99984	100278	964-964	0.500000	59.8
ycf15-trnV-GAC_p5	ycf15-trnV-GAC	5	TGACCTCCTTGCTTCTCTCA	CCTCAGCCTACGGGGTATTA	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.020826	99984	100278	897-897	0.500000	59.8
trnA-UGC_p1	trnA-UGC	1	TGATTTACTTCACGGGCGAG	ATTCGGGAATCTCCGGATCT	20	20	50.0	50.0	58.0	58.0	0.0	3.6	0.021487	103080	103795	1109-1276	1.000000	90.6
trnA-UGC_p2	trnA-UGC	2	TGATTTACTTCACGGGCGAG	ATGTTTCAGTTCGCCAGGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.023326	103080	103795	1176-1343	1.000000	90.2
trnA-UGC_p3	trnA-UGC	3	TGATTTACTTCACGGGCGAG	TTTTCACGGTTTAGGCTGCT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.028923	103080	103795	1257-1424	1.000000	89.5
trnA-UGC_p4	trnA-UGC	4	TGATTTACTTCACGGGCGAG	AGTGATGCTTTCGGCTACTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.020318	103080	103795	1355-1522	1.000000	88.9
trnA-UGC_p5	trnA-UGC	5	TGAATCGCCCCATCTATCCT	AGTGATGCTTTCGGCTACTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.045763	103080	103795	1733-1733	0.500000	59.6
ycf1_p1	ycf1	1	GATTTCCTTGAGACGGTCCC	AGTTGTAGAGCGATTCTGCG	20	20	55.0	50.0	58.0	58.1	4.1	0.0	0.110955	110776	110895	578-578	0.500000	59.1
ycf1_p2	ycf1	2	GGATTTCCTTGAGACGGTCC	AGTTGTAGAGCGATTCTGCG	20	20	55.0	50.0	58.0	58.1	4.1	0.0	0.110955	110776	110895	579-579	0.500000	59.1
ycf1_p3	ycf1	3	GGGATTTCCTTGAGACGGTC	AGTTGTAGAGCGATTCTGCG	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.110955	110776	110895	580-580	0.500000	59.1
ycf1_p4	ycf1	4	GAGACGGTCCCCAAATAACC	AGTTGTAGAGCGATTCTGCG	20	20	55.0	50.0	58.0	58.1	4.1	0.0	0.119471	110776	110895	569-569	0.500000	59.0
ycf1_p5	ycf1	5	CGCCTTTGAATACGGATGGA	AGTTGTAGAGCGATTCTGCG	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.137262	110776	110895	543-543	0.500000	58.9
ndhE-psaC_p1	ndhE-psaC	1	CTGAAGCCGCTATTGGACTC	ACATCCGTCCCAAGGTATCA	20	20	55.0	50.0	58.4	58.1	0.0	0.0	0.549515	119265	119448	378-428	1.000000	83.3
ndhE-psaC_p2	ndhE-psaC	2	CCGCTATTGGACTCGCTATT	ACATCCGTCCCAAGGTATCA	20	20	50.0	50.0	57.6	58.1	0.0	0.0	0.566410	119265	119448	372-422	1.000000	83.3
ndhE-psaC_p3	ndhE-psaC	3	CTGAAGCCGCTATTGGACTC	GGACACATTGAGTACACCCG	20	20	55.0	55.0	58.4	58.3	0.0	0.0	0.701633	119265	119448	331-381	1.000000	83.2
ndhE-psaC_p4	ndhE-psaC	4	CTGAAGCCGCTATTGGACTC	TTTGGCTTTACATCCGTCCC	20	20	55.0	50.0	58.4	58.2	0.0	0.0	0.594665	119265	119448	387-437	1.000000	82.8
ndhE-psaC_p5	ndhE-psaC	5	CCGCTATTGGACTCGCTATT	TTTGGCTTTACATCCGTCCC	20	20	50.0	50.0	57.6	58.2	0.0	0.0	0.611560	119265	119448	381-431	1.000000	82.8
trnA-UGC_p1	trnA-UGC	1	ATTCGGGAATCTCCGGATCT	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	58.0	58.0	3.6	0.0	0.021487	131705	132420	1109-1276	1.000000	90.6
trnA-UGC_p2	trnA-UGC	2	ATGTTTCAGTTCGCCAGGTT	TGATTTACTTCACGGGCGAG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.023326	131705	132420	1176-1343	1.000000	90.2
trnA-UGC_p3	trnA-UGC	3	TTTTCACGGTTTAGGCTGCT	TGATTTACTTCACGGGCGAG	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.028923	131705	132420	1257-1424	1.000000	89.5
trnA-UGC_p4	trnA-UGC	4	AGTGATGCTTTCGGCTACTG	TGATTTACTTCACGGGCGAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.020318	131705	132420	1355-1522	1.000000	88.9
trnA-UGC_p5	trnA-UGC	5	AGTGATGCTTTCGGCTACTG	TGAATCGCCCCATCTATCCT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.045763	131705	132420	1733-1733	0.500000	59.6
trnI-GAU_p1	trnI-GAU	1	TCTGACTCCTTCATGCATGC	CCGCGAATCCTCTTGAAAGA	20	20	50.0	50.0	58.0	58.0	22.2	8.2	0.044249	132496	133310	1633-1633	0.500000	59.6
trnI-GAU_p2	trnI-GAU	2	TGACTCCTTCATGCATGCTC	CCGCGAATCCTCTTGAAAGA	20	20	50.0	50.0	58.0	58.0	22.2	8.2	0.044249	132496	133310	1631-1631	0.500000	59.6
trnI-GAU_p3	trnI-GAU	3	GCGCCAGGGAAAAGAATAGA	CCGCGAATCCTCTTGAAAGA	20	20	50.0	50.0	58.0	58.0	0.0	8.2	0.048532	132496	133310	1660-1660	0.500000	59.6
trnI-GAU_p4	trnI-GAU	4	CTGACTCCTTCATGCATGCT	CCGCGAATCCTCTTGAAAGA	20	20	50.0	50.0	58.0	58.0	22.2	8.2	0.049963	132496	133310	1632-1632	0.500000	59.6
trnI-GAU_p5	trnI-GAU	5	TGACTCCTTCATGCATGCTC	AACTCCAAAAACCCGTCCTC	20	20	50.0	50.0	58.0	58.0	22.2	0.0	0.057048	132496	133310	1352-1352	0.500000	59.5
ycf15-rps12_p1	ycf15-rps12	1	GTACCGTGAGAGAAGCAAGG	TACTCCGACAGCATCTAGGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.019671	135709	136791	1363-1363	0.500000	59.8
ycf15-rps12_p2	ycf15-rps12	2	GTACCGTGAGAGAAGCAAGG	AGCGATTCACATGGCATCAT	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.025275	135709	136791	1445-1445	0.500000	59.8
ycf15-rps12_p3	ycf15-rps12	3	GAATAGAACACGCTGAGCCA	TACTCCGACAGCATCTAGGG	20	20	50.0	55.0	58.0	58.0	0.0	0.0	0.027485	135709	136791	1754-1754	0.500000	59.8
ycf15-rps12_p4	ycf15-rps12	4	CCGGCTTAGTGAGTCCTTTC	TACTCCGACAGCATCTAGGG	20	20	55.0	55.0	58.0	58.0	0.0	0.0	0.028880	135709	136791	1464-1464	0.500000	59.8
ycf15-rps12_p5	ycf15-rps12	5	GTACCGTGAGAGAAGCAAGG	TCTACAACGCACTAGAACGC	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.029193	135709	136791	1400-1400	0.500000	59.8
rpl2_p1	rpl2	1	ACTTAATAGCATGGCGATACA	TGTAAAGACGAAGAAAGAAATTC	21	23	38.1	30.4	54.9	53.4	0.0	0.0	11.742856	149250	150530	1339-1534	1.000000	50.3
rpl2_p2	rpl2	2	ACTTAATAGCATGGCGATACA	TTTGTAAAGACGAAGAAAGAAA	21	22	38.1	27.3	54.9	52.2	0.0	0.0	11.859434	149250	150530	1341-1536	1.000000	50.3
rpl2_p3	rpl2	3	ACTTAATAGCATGGCGATACA	TTTTGTAAAGACGAAGAAAGAA	21	22	38.1	27.3	54.9	52.2	0.0	0.0	11.859434	149250	150530	1342-1537	1.000000	50.3
rpl2_p4	rpl2	4	ACTTAATAGCATGGCGATACA	TTTTTGTAAAGACGAAGAAAGA	21	22	38.1	27.3	54.9	52.2	0.0	0.0	11.859434	149250	150530	1343-1538	1.000000	50.3
rpl2_p5	rpl2	5	ACTTAATAGCATGGCGATACA	TTGTAAAGACGAAGAAAGAAATTC	21	24	38.1	29.2	54.9	54.1	0.0	0.0	11.968119	149250	150530	1340-1535	1.000000	50.3