Hotspot

ndhF

SSC · 102389-104605 · 2217 bp

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Pi 0.00135

MarkerSeek score 58.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 3
pi: 0.00135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	TGCATTTTGATGAGCCTCGT	AGGTTTGGTGAATCGGAAACA	45.0	42.9	58.2	58.1	2347 bp	1.0	70.2
ndhF_p2	TGCATTTTGATGAGCCTCGT	GGTTTGGTGAATCGGAAACA	45.0	45.0	58.2	56.5	2346 bp	1.0	67.1
ndhF_p3	TGCATTTTGATGAGCCTCGT	GGTTTGGTGAATCGGAAACAA	45.0	42.9	58.2	57.3	2346 bp	1.0	64.9
ndhF_p4	TGCATTTTGATGAGCCTCGT	AGGTTTGGTGAATCGGAAAC	45.0	45.0	58.2	56.2	2347 bp	1.0	64.7
ndhF_p5	TTGCATTTTGATGAGCCTCG	AGGTTTGGTGAATCGGAAACA	45.0	42.9	57.1	58.1	2348 bp	1.0	64.2