Hotspot

ndhD

SSC · 107518-109020 · 1503 bp

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Pi 0.000665

MarkerSeek score 59.10

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1503
variable_sites: 1
pi: 0.000665
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGGAATATAGAGCGGACTGGT	GGGCTTTTCTGGTCAAAACG	47.6	50.0	58.0	57.9	1608 bp	1.0	70.6
ndhD_p2	TGGAATATAGAGCGGACTGGT	TTTACCGAAGAAGCCTGTGC	47.6	50.0	58.0	58.5	1645 bp	1.0	67.9
ndhD_p3	CCACTCTGGAATATAGAGCGG	GGGCTTTTCTGGTCAAAACG	52.4	50.0	57.4	57.9	1614 bp	1.0	66.3
ndhD_p4	CTGGAATATAGAGCGGACTGG	GGGCTTTTCTGGTCAAAACG	52.4	50.0	57.4	57.9	1609 bp	1.0	66.3
ndhD_p5	TGGAATATAGAGCGGACTGGT	GGCTTTTCTGGTCAAAACGT	47.6	45.0	58.0	57.1	1607 bp	1.0	64.8