Hotspot

infA-rps8

LSC · 75636-75714 · 79 bp

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Pi 0.0127

MarkerSeek score 59.80

Primer available yes

Label infA-rps8

Feature Metadata

label_name: infA-rps8
feature_type: igs
region: LSC
length_bp: 79
variable_sites: 1
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA-rps8_p1	GGGTAGGGCTTCCGTAACTA	ATTCCTAAGGTTTTGGGCGG	55.0	50.0	58.2	58.2	286 bp	1.0	77.3
infA-rps8_p2	GGGTAGGGCTTCCGTAACTA	CCTAAGGTTTTGGGCGGAAT	55.0	50.0	58.2	58.2	283 bp	1.0	77.3
infA-rps8_p3	GAACATTCCGTTGGGTAGGG	ATTCCTAAGGTTTTGGGCGG	55.0	50.0	58.3	58.2	298 bp	1.0	77.0
infA-rps8_p4	GAACATTCCGTTGGGTAGGG	CCTAAGGTTTTGGGCGGAAT	55.0	50.0	58.3	58.2	295 bp	1.0	77.0
infA-rps8_p5	GGAACATTCCGTTGGGTAGG	CCTAAGGTTTTGGGCGGAAT	55.0	50.0	58.3	58.2	296 bp	1.0	77.0