Hotspot

atpB-rbcL

LSC · 53143-53949 · 807 bp

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Pi 0

MarkerSeek score 58.90

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 807
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TATCCAGCACGGGTCCAATA	ACTCCGGGGTGTAGTAAGTC	50.0	55.0	58.2	58.2	988-989 bp	1.0	77.2
atpB-rbcL_p2	TATCCAGCACGGGTCCAATA	CTCCGGGGTGTAGTAAGTCA	50.0	55.0	58.2	58.2	987-988 bp	1.0	77.2
atpB-rbcL_p3	CCAGCACGGGTCCAATAATT	ACTCCGGGGTGTAGTAAGTC	50.0	55.0	58.2	58.2	985-986 bp	1.0	76.9
atpB-rbcL_p4	CCAGCACGGGTCCAATAATT	CTCCGGGGTGTAGTAAGTCA	50.0	55.0	58.2	58.2	984-985 bp	1.0	76.9
atpB-rbcL_p5	CCGGGGGAAAAGTGATATCC	ACTCCGGGGTGTAGTAAGTC	55.0	55.0	57.7	58.2	1003-1004 bp	1.0	76.7