Hotspot

trnD-GUC-psbM

LSC · 15062-16118 · 1057 bp

Back to Agenium

Pi 0.000946

MarkerSeek score 59.30

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1057
variable_sites: 1
pi: 0.000946
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	AATAGGCATGCCATACACCC	AGGCAGTAGGGACTAGAATGA	50.0	47.6	57.7	57.3	1216-1218 bp	1.0	64.2
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	AGGCAGTAGGGACTAGAATGA	50.0	47.6	58.5	57.3	1182-1184 bp	1.0	62.7
trnD-GUC-psbM_p3	AATAGGCATGCCATACACCC	AAGGCAGTAGGGACTAGAATGA	50.0	45.5	57.7	58.0	1217-1219 bp	1.0	61.7
trnD-GUC-psbM_p4	AATAGGCATGCCATACACCC	AGGCAGTAGGGACTAGAATGAA	50.0	45.5	57.7	58.0	1216-1218 bp	1.0	61.7
trnD-GUC-psbM_p5	AATAGGCATGCCATACACCC	GGCAGTAGGGACTAGAATGAAC	50.0	50.0	57.7	57.9	1215-1217 bp	1.0	61.2