Hotspot

matK-trnK-UUU

LSC · 3242-3937 · 696 bp

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Pi 0.00144
MarkerSeek score 59.60
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
696
variable_sites
1
pi
0.00144
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 CCATAATCACGAGCAAATGCA GAGCGTCCGGTGTAATTTCA 42.9 50.0 57.3 58.3 898 bp 1.0 64.3
matK-trnK-UUU_p2 ACGAAGTAGTGTTGACGAGA GAGCGTCCGGTGTAATTTCA 45.0 50.0 56.3 58.3 847 bp 1.0 64.1
matK-trnK-UUU_p3 CCATAATCACGAGCAAATGCA AGCGTCCGGTGTAATTTCAT 42.9 45.0 57.3 57.6 897 bp 1.0 63.3
matK-trnK-UUU_p4 ACGAAGTAGTGTTGACGAGA AGCGTCCGGTGTAATTTCAT 45.0 45.0 56.3 57.6 846 bp 1.0 63.1
matK-trnK-UUU_p5 CCATAATCACGAGCAAATGCA ATGTTTTGACAGGAGCGTCC 42.9 50.0 57.3 58.5 910 bp 1.0 62.7