Hotspot

psbA-trnK-UUU

LSC · 1157-1402 · 246 bp

Back to Agenium

Pi 0.00407

MarkerSeek score 44.50

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: LSC
length_bp: 246
variable_sites: 1
pi: 0.00407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	AAGACGGTTTTCGGTGCTAG	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.2	58.6	380 bp	1.0	73.7
psbA-trnK-UUU_p2	AAAGACGGTTTTCGGTGCTA	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.8	58.6	381 bp	1.0	73.4
psbA-trnK-UUU_p3	TAAAGACGGTTTTCGGTGCT	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.8	58.6	382 bp	1.0	73.4
psbA-trnK-UUU_p4	AACACCGAACCATCCAATGT	ACTCCATCCGACTAGTTCCG	45.0	55.0	57.7	58.6	401 bp	1.0	73.0
psbA-trnK-UUU_p5	GCTTGTACTTTCGCGTCTCT	ACTCCATCCGACTAGTTCCG	50.0	55.0	58.3	58.6	332 bp	1.0	73.0