Hotspot

ycf1

IRb · 111558-112474 · 917 bp

Back to Agave

Pi 0.000623
MarkerSeek score 46.10
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
IRb
length_bp
917
variable_sites
1
pi
0.000623
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 TGGTATCATTGACATAAGAGATGTC AATATATGGGAGGTGGGCGA 36.0 50.0 56.5 58.0 1125-1135 bp 1.0 40.8
ycf1_p2 TGGTATCATTGACATAAGAGATGT AATATATGGGAGGTGGGCGA 33.3 50.0 55.4 58.0 1125-1135 bp 1.0 40.8
ycf1_p3 TGGTATCATTGACATAAGAGATGTC ATATATGGGAGGTGGGCGAG 36.0 55.0 56.5 58.4 1124-1134 bp 1.0 40.8
ycf1_p4 TGGTATCATTGACATAAGAGATGT ATATATGGGAGGTGGGCGAG 33.3 55.0 55.4 58.4 1124-1134 bp 1.0 40.8
ycf1_p5 GGTATCATTGACATAAGAGATGTC AATATATGGGAGGTGGGCGA 37.5 50.0 54.9 58.0 1124-1134 bp 1.0 40.8