Hotspot

rbcL

LSC · 56964-58406 · 1443 bp

Back to Agave

Pi 0
MarkerSeek score 30.60
Primer available yes
Label rbcL

Feature Metadata

label_name
rbcL
feature_type
gene
region
LSC
length_bp
1443
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
30.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL_p1 TGAGTTGTAGGGAGGGACTT ACTGAATTATGCGCGCTTAG 50.0 45.0 57.3 56.7 1486 bp 1.0 63.8
rbcL_p2 TGAGTTGTAGGGAGGGACTT CTGAATTATGCGCGCTTAGT 50.0 45.0 57.3 56.7 1485 bp 1.0 63.8
rbcL_p3 TGAGTTGTAGGGAGGGACTT ACTGAATTATGCGCGCTTAGT 50.0 42.9 57.3 58.4 1486 bp 1.0 62.7
rbcL_p4 TCATGAGTTGTAGGGAGGGA ACTGAATTATGCGCGCTTAG 50.0 45.0 57.1 56.7 1489 bp 1.0 62.0
rbcL_p5 TCATGAGTTGTAGGGAGGGA CTGAATTATGCGCGCTTAGT 50.0 45.0 57.1 56.7 1488 bp 1.0 62.0