Hotspot

petB

LSC · 76953-78410 · 1458 bp

Back to Agave

Pi 0
MarkerSeek score 45.50
Primer available yes
Label petB

Feature Metadata

label_name
petB
feature_type
gene
region
LSC
length_bp
1458
variable_sites
0
pi
0
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
45.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB_p1 TCTGGTAGTTCGACTGTGGA ACTATTAGTCCTTCCCGATATGA 50.0 39.1 57.7 55.8 1584-1586 bp 1.0 40.1
petB_p2 TCTGGTAGTTCGACTGTGGA TGAAATACTATTAGTCCTTCCCGA 50.0 37.5 57.7 56.7 1590-1592 bp 1.0 40.1
petB_p3 TCTGGTAGTTCGACTGTGGA AGTCCTTCCCGATATGATAA 50.0 40.0 57.7 52.6 1578-1580 bp 1.0 40.1
petB_p4 TCTGGTAGTTCGACTGTGGA ATTAGTCCTTCCCGATATGA 50.0 40.0 57.7 52.6 1581-1583 bp 1.0 40.1
petB_p5 TCTGGTAGTTCGACTGTGGA TTAGTCCTTCCCGATATGAT 50.0 40.0 57.7 52.6 1580-1582 bp 1.0 40.1