Hotspot

ndhG

SSC · 120607-121137 · 531 bp

Back to Agave

Pi 0
MarkerSeek score 28.70
Primer available yes
Label ndhG

Feature Metadata

label_name
ndhG
feature_type
gene
region
SSC
length_bp
531
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
28.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG_p1 TGGTTGACTAGAATCGAGCA ACCCTTATTTTTCCTGGACT 45.0 40.0 56.3 53.8 716 bp 1.0 40.0
ndhG_p2 TGGTTGACTAGAATCGAGCAA ACCCTTATTTTTCCTGGACT 42.9 40.0 57.0 53.8 716 bp 1.0 40.0
ndhG_p3 TTGGTTGACTAGAATCGAGCA ACCCTTATTTTTCCTGGACT 42.9 40.0 57.0 53.8 717 bp 1.0 40.0
ndhG_p4 TTGGTTGACTAGAATCGAGCAA ACCCTTATTTTTCCTGGACT 40.9 40.0 57.7 53.8 717 bp 1.0 40.0
ndhG_p5 TTTGGTTGACTAGAATCGAGCA ACCCTTATTTTTCCTGGACT 40.9 40.0 57.7 53.8 718 bp 1.0 40.0