Hotspot

ccsA

SSC · 116649-117617 · 969 bp

Back to Agave

Pi 0
MarkerSeek score 30.40
Primer available yes
Label ccsA

Feature Metadata

label_name
ccsA
feature_type
gene
region
SSC
length_bp
969
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
30.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA_p1 GAGTGGCGGCATACTCTAAA AGGGTTCTCAAAAACTCGCA 50.0 45.0 57.4 57.7 1116 bp 1.0 72.5
ccsA_p2 GAGTGGCGGCATACTCTAAA GGGTTCTCAAAAACTCGCAC 50.0 50.0 57.4 57.6 1115 bp 1.0 72.0
ccsA_p3 AGTGGCGGCATACTCTAAAA AGGGTTCTCAAAAACTCGCA 45.0 45.0 56.9 57.7 1115 bp 1.0 68.7
ccsA_p4 AGTGGCGGCATACTCTAAAAG AGGGTTCTCAAAAACTCGCA 47.6 45.0 58.1 57.7 1115 bp 1.0 68.5
ccsA_p5 GTGGCGGCATACTCTAAAAGA AGGGTTCTCAAAAACTCGCA 47.6 45.0 58.1 57.7 1114 bp 1.0 68.5