Hotspot

ccsA-ndhD

SSC · 117618-117845 · 228 bp

Back to Agave

Pi 0.0125
MarkerSeek score 48.20
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
228
variable_sites
5
pi
0.0125
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 ATTCGGCACTTGTAGCTTCT TTTCGTTCTCTCGCTGTCAG 45.0 50.0 57.2 57.9 407-409 bp 1.0 76.0
ccsA-ndhD_p2 ACAAATTCGGCACTTGTAGC TTTCGTTCTCTCGCTGTCAG 45.0 50.0 57.0 57.9 411-413 bp 1.0 74.2
ccsA-ndhD_p3 AATTCGGCACTTGTAGCTTCT TTTCGTTCTCTCGCTGTCAG 42.9 50.0 57.9 57.9 408-410 bp 1.0 73.7
ccsA-ndhD_p4 ATTCGGCACTTGTAGCTTCT CGATTTCGTTCTCTCGCTGT 45.0 50.0 57.2 58.4 410-412 bp 1.0 73.3
ccsA-ndhD_p5 ATTCGGCACTTGTAGCTTCT TTCTCTCGCTGTCAGTTGAC 45.0 50.0 57.2 57.6 402-404 bp 1.0 73.0