Hotspot

rpl20-rps12

LSC · 70974-71741 · 768 bp

Back to Agave

Pi 0.00112
MarkerSeek score 46.70
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
768
variable_sites
2
pi
0.00112
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TTCTGTTGAGTAGCGGTTCG CAGCGTAGGGGAACATGTAC 50.0 55.0 57.9 58.1 913-916 bp 1.0 79.4
rpl20-rps12_p2 TTCTGTTGAGTAGCGGTTCG AATATCACGAAATCCCCCGC 50.0 50.0 57.9 58.1 949-952 bp 1.0 79.1
rpl20-rps12_p3 GGTTCGGGTAAGCCTTGAAT CAGCGTAGGGGAACATGTAC 50.0 55.0 57.9 58.1 899-902 bp 1.0 78.9
rpl20-rps12_p4 TTCTGTTGAGTAGCGGTTCG AGCGTAGGGGAACATGTACT 50.0 50.0 57.9 58.1 912-915 bp 1.0 78.9
rpl20-rps12_p5 TTCTGTTGAGTAGCGGTTCG TCAGCGTAGGGGAACATGTA 50.0 50.0 57.9 58.1 914-917 bp 1.0 78.8