Hotspot

rbcL-accD

LSC · 58407-59134 · 728 bp

Back to Agave

Pi 0.00432
MarkerSeek score 46.00
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
728
variable_sites
8
pi
0.00432
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GCAAATGGAGCCCTGAACTA CACCAGTATGTCCAACAGCA 50.0 50.0 57.9 57.8 938-940 bp 1.0 78.2
rbcL-accD_p2 CGCTGCTTGTGAAGTATGGA CACCAGTATGTCCAACAGCA 50.0 50.0 58.3 57.8 916-918 bp 1.0 77.0
rbcL-accD_p3 AAATGGAGCCCTGAACTAGC CACCAGTATGTCCAACAGCA 50.0 50.0 57.6 57.8 936-938 bp 1.0 75.8
rbcL-accD_p4 GCAAATGGAGCCCTGAACTA CCACCAGTATGTCCAACAGC 50.0 55.0 57.9 58.5 939-941 bp 1.0 75.3
rbcL-accD_p5 CGCTGCTTGTGAAGTATGGA CCACCAGTATGTCCAACAGC 50.0 55.0 58.3 58.5 917-919 bp 1.0 74.1