Hotspot

rpl32-ndhF

Genome · 138570-139911 · 1342 bp

Back to Agapetes

Pi 0.0508

MarkerSeek score 74.30

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: Genome
length_bp: 1342
variable_sites: 67
pi: 0.0508
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	AGCGTGTCTACCGATTTCAC	AACAGGTCGTGTGAACCAAA	50.0	45.0	58.0	57.9	2584 bp	0.5	59.0
rpl32-ndhF_p2	AGCGTGTCTACCGATTTCAC	ACAGGTCGTGTGAACCAAAA	50.0	45.0	58.0	57.9	2583 bp	0.5	59.0
rpl32-ndhF_p3	CAGCGTGTCTACCGATTTCA	AACAGGTCGTGTGAACCAAA	50.0	45.0	58.0	57.9	2585 bp	0.5	59.0
rpl32-ndhF_p4	CAGCGTGTCTACCGATTTCA	ACAGGTCGTGTGAACCAAAA	50.0	45.0	58.0	57.9	2584 bp	0.5	59.0
rpl32-ndhF_p5	CGAAACACATTTCCCCAACG	ACAGGTCGTGTGAACCAAAA	50.0	45.0	58.0	57.9	1968 bp	0.5	58.7