Hotspot

rps16-rrn16

Genome · 108806-114471 · 5666 bp

Back to Agapetes

Pi 0.0944

MarkerSeek score 63.80

Primer available yes

Label rps16-rrn16

Feature Metadata

label_name: rps16-rrn16
feature_type: igs
region: Genome
length_bp: 5666
variable_sites: 322
pi: 0.0944
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.60
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-rrn16_p1	AATAAGCCGCTCCATGAAGG	TCATAGAGCGCCCTGTGATA	50.0	50.0	58.0	58.0	1459-2129 bp	1.0	85.8
rps16-rrn16_p2	AAGACAACCAGAGACGGGTA	TTGCACAAACAATGCTTGCA	50.0	40.0	58.0	58.0	1174 bp	0.5	59.8
rps16-rrn16_p3	AATAAGCCGCTCCATGAAGG	ATCCAAAAACCGCCAGTTCT	50.0	45.0	58.0	58.0	1571 bp	0.5	59.7
rps16-rrn16_p4	AATAAGCCGCTCCATGAAGG	TTGCACAAACAATGCTTGCA	50.0	40.0	58.0	58.0	1100 bp	0.5	59.6
rps16-rrn16_p5	GGCAGCGAATCGAACAAAAA	TCATAGAGCGCCCTGTGATA	45.0	50.0	58.0	58.0	945 bp	0.5	59.6