Hotspot

rps12-trnV-GAC

Genome · 103487-105060 · 1574 bp

Back to Agapetes

Pi 0.0344

MarkerSeek score 68.00

Primer available yes

Label rps12-trnV-GAC

Feature Metadata

label_name: rps12-trnV-GAC
feature_type: igs
region: Genome
length_bp: 1574
variable_sites: 54
pi: 0.0344
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-trnV-GAC_p1	TGGCAACTTTACGTAAGGCA	GACTTTCACCACGTCAAGGT	45.0	50.0	57.7	58.1	1663-1667 bp	1.0	80.4
rps12-trnV-GAC_p2	TGGCAACTTTACGTAAGGCA	TGACTTTCACCACGTCAAGG	45.0	50.0	57.7	58.1	1664-1668 bp	1.0	80.4
rps12-trnV-GAC_p3	ACGTAAGGCAGAGTTTGGTT	GACTTTCACCACGTCAAGGT	45.0	50.0	57.4	58.1	1653-1657 bp	1.0	77.4
rps12-trnV-GAC_p4	ACGTAAGGCAGAGTTTGGTT	TGACTTTCACCACGTCAAGG	45.0	50.0	57.4	58.1	1654-1658 bp	1.0	77.4
rps12-trnV-GAC_p5	TGGCAACTTTACGTAAGGCA	AGGTGACACTCTACCACTGA	45.0	50.0	57.7	57.3	1647-1651 bp	1.0	75.4