Hotspot

rpl36-rps8

Genome · 86443-87343 · 901 bp

Back to Agapetes

Pi 0.0547

MarkerSeek score 79.40

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: Genome
length_bp: 901
variable_sites: 49
pi: 0.0547
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	GTTTATGCCTGGGGTTGGAA	AATGACAGATCGAGAGGCTC	50.0	50.0	58.1	56.8	1065-1198 bp	1.0	73.9
rpl36-rps8_p2	TTTATGCCTGGGGTTGGAAC	AATGACAGATCGAGAGGCTC	50.0	50.0	58.1	56.8	1064-1197 bp	1.0	73.9
rpl36-rps8_p3	CCTACGAATTAGGCGGCATT	AATGACAGATCGAGAGGCTC	50.0	50.0	58.1	56.8	1022-1155 bp	1.0	73.6
rpl36-rps8_p4	GTTTATGCCTGGGGTTGGAA	TCGACTAAAAGGAATTGGGGG	50.0	47.6	58.1	57.6	1047-1180 bp	1.0	72.5
rpl36-rps8_p5	TTTATGCCTGGGGTTGGAAC	TCGACTAAAAGGAATTGGGGG	50.0	47.6	58.1	57.6	1046-1179 bp	1.0	72.5