Hotspot

trnM-CAU-psaI

Genome · 17841-20130 · 2290 bp

Back to Agapetes

Pi 0.029
MarkerSeek score 68.90
Primer available yes
Label trnM-CAU-psaI

Feature Metadata

label_name
trnM-CAU-psaI
feature_type
igs
region
Genome
length_bp
2290
variable_sites
66
pi
0.029
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnM-CAU-psaI_p1 TTGAACCAATGACTCTCGCC GCCGGAAATACTAGGCCTAC 50.0 55.0 58.2 57.5 2406-2488 bp 1.0 77.4
trnM-CAU-psaI_p2 TCTCGCCGTATGAAAGCAAT GCCGGAAATACTAGGCCTAC 45.0 55.0 57.7 57.5 2393-2475 bp 1.0 76.5
trnM-CAU-psaI_p3 CTCTCGCCGTATGAAAGCAA GCCGGAAATACTAGGCCTAC 50.0 55.0 58.4 57.5 2394-2476 bp 1.0 76.1
trnM-CAU-psaI_p4 CAATGACTCTCGCCGTATGA GCCGGAAATACTAGGCCTAC 50.0 55.0 57.5 57.5 2400-2482 bp 1.0 75.0
trnM-CAU-psaI_p5 CCAATGACTCTCGCCGTATG GCCGGAAATACTAGGCCTAC 55.0 55.0 58.5 57.5 2401-2483 bp 1.0 74.9