Hotspot

matK-trnK-UUU

Genome · 2246-2959 · 714 bp

Back to Agapetes

Pi 0.0266

MarkerSeek score 70.20

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: Genome
length_bp: 714
variable_sites: 19
pi: 0.0266
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	ACGATCATGAGCAAGTGCAT	CGATTCGGGTTGCTAACTCA	45.0	50.0	58.0	58.0	862-863 bp	1.0	81.5
matK-trnK-UUU_p2	ACGATCATGAGCAAGTGCAT	TCGGGTTGCTAACTCAATGG	45.0	50.0	58.0	57.9	858-859 bp	1.0	80.7
matK-trnK-UUU_p3	ACGATCATGAGCAAGTGCAT	CGGGTTGCTAACTCAATGGT	45.0	50.0	58.0	58.2	857-858 bp	1.0	80.2
matK-trnK-UUU_p4	ACCACGATCATGAGCAAGTG	CGATTCGGGTTGCTAACTCA	50.0	50.0	58.3	58.0	865-866 bp	1.0	79.7
matK-trnK-UUU_p5	ACGATCATGAGCAAGTGCAT	TCGATTCGGGTTGCTAACTC	45.0	50.0	58.0	57.7	863-864 bp	1.0	79.3