primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	matK-trnK-UUU	1	ACGATCATGAGCAAGTGCAT	CGATTCGGGTTGCTAACTCA	20	20	45.0	50.0	58.0	58.0	12.1	0.0	0.038687	2246	2959	862-863	1.000000	81.5
matK-trnK-UUU_p2	matK-trnK-UUU	2	ACGATCATGAGCAAGTGCAT	TCGGGTTGCTAACTCAATGG	20	20	45.0	50.0	58.0	57.9	12.1	0.0	0.142054	2246	2959	858-859	1.000000	80.7
matK-trnK-UUU_p3	matK-trnK-UUU	3	ACGATCATGAGCAAGTGCAT	CGGGTTGCTAACTCAATGGT	20	20	45.0	50.0	58.0	58.2	12.1	0.0	0.212888	2246	2959	857-858	1.000000	80.2
matK-trnK-UUU_p4	matK-trnK-UUU	4	ACCACGATCATGAGCAAGTG	CGATTCGGGTTGCTAACTCA	20	20	50.0	50.0	58.3	58.0	12.1	0.0	0.274082	2246	2959	865-866	1.000000	79.7
matK-trnK-UUU_p5	matK-trnK-UUU	5	ACGATCATGAGCAAGTGCAT	TCGATTCGGGTTGCTAACTC	20	20	45.0	50.0	58.0	57.7	12.1	6.2	0.323065	2246	2959	863-864	1.000000	79.3
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	ACCAATTTCGCCATATCCCC	20	20	55.0	50.0	57.9	58.0	0.0	0.0	0.137364	8176	8909	784-822	1.000000	81.5
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GCGATGCTCTAACCTCTGAG	TAGCGTCTACCAATTTCGCC	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.202173	8176	8909	792-830	1.000000	80.9
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GGAATCGAACCGATGACCAT	ACCAATTTCGCCATATCCCC	20	20	50.0	50.0	57.8	58.0	15.5	0.0	0.245117	8176	8909	816-854	1.000000	80.5
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GGAATCGAACCGATGACCAT	TAGCGTCTACCAATTTCGCC	20	20	50.0	50.0	57.8	58.1	15.5	0.0	0.309926	8176	8909	824-862	1.000000	80.0
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	20	20	55.0	50.0	57.9	58.2	0.0	10.2	0.372548	8176	8909	798-836	1.000000	79.6
trnM-CAU-psaI_p1	trnM-CAU-psaI	1	TTGAACCAATGACTCTCGCC	GCCGGAAATACTAGGCCTAC	20	20	50.0	55.0	58.2	57.5	0.0	8.9	0.643885	17841	20130	2406-2488	1.000000	77.4
trnM-CAU-psaI_p2	trnM-CAU-psaI	2	TCTCGCCGTATGAAAGCAAT	GCCGGAAATACTAGGCCTAC	20	20	45.0	55.0	57.7	57.5	0.0	8.9	0.761823	17841	20130	2393-2475	1.000000	76.5
trnM-CAU-psaI_p3	trnM-CAU-psaI	3	CTCTCGCCGTATGAAAGCAA	GCCGGAAATACTAGGCCTAC	20	20	50.0	55.0	58.4	57.5	0.0	8.9	0.811868	17841	20130	2394-2476	1.000000	76.1
trnM-CAU-psaI_p4	trnM-CAU-psaI	4	CAATGACTCTCGCCGTATGA	GCCGGAAATACTAGGCCTAC	20	20	50.0	55.0	57.5	57.5	0.0	8.9	0.944315	17841	20130	2400-2482	1.000000	75.0
trnM-CAU-psaI_p5	trnM-CAU-psaI	5	CCAATGACTCTCGCCGTATG	GCCGGAAATACTAGGCCTAC	20	20	55.0	55.0	58.5	57.5	0.0	8.9	0.960625	17841	20130	2401-2483	1.000000	74.9
trnP-UGG-trnW-CCA_p1	trnP-UGG-trnW-CCA	1	TACAAAATGTCGCGGGTTCA	GTTTTGGAGACCCACGTTCT	20	20	45.0	50.0	58.1	58.0	6.1	0.0	0.156557	33511	33696	246-262	1.000000	84.3
trnP-UGG-trnW-CCA_p2	trnP-UGG-trnW-CCA	2	TACAAAATGTCGCGGGTTCA	TTTTGGAGACCCACGTTCTG	20	20	45.0	50.0	58.1	58.0	6.1	0.0	0.157935	33511	33696	245-261	1.000000	84.3
trnP-UGG-trnW-CCA_p3	trnP-UGG-trnW-CCA	3	CAAAATGTCGCGGGTTCAAA	GTTTTGGAGACCCACGTTCT	20	20	45.0	50.0	57.9	58.0	6.1	0.0	0.163741	33511	33696	244-260	1.000000	84.3
trnP-UGG-trnW-CCA_p4	trnP-UGG-trnW-CCA	4	TACAAAATGTCGCGGGTTCA	AGGAAATGAAATCGCGCTCT	20	20	45.0	45.0	58.1	58.0	6.1	5.6	0.149567	33511	33696	307-307	0.500000	58.8
trnP-UGG-trnW-CCA_p5	trnP-UGG-trnW-CCA	5	CAAAATGTCGCGGGTTCAAA	AGGAAATGAAATCGCGCTCT	20	20	45.0	45.0	57.9	58.0	6.1	5.6	0.156752	33511	33696	305-305	0.500000	58.7
trnfM-CAU-atpI_p1	trnfM-CAU-atpI	1	CCAGACTACTCTACTCCCCG	TTTTGCAACTTTAGCTGCGG	20	20	60.0	45.0	58.0	57.9	0.0	0.0	0.162648	66857	67255	474-480	1.000000	81.5
trnfM-CAU-atpI_p2	trnfM-CAU-atpI	2	AAGGTTATGAGCCTTGCGAG	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.162578	66857	67255	497-503	1.000000	81.4
trnfM-CAU-atpI_p3	trnfM-CAU-atpI	3	TTGCGAGCTACCAGACTACT	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	58.2	57.9	0.0	0.0	0.297246	66857	67255	484-490	1.000000	80.4
trnfM-CAU-atpI_p4	trnfM-CAU-atpI	4	CTTGCGAGCTACCAGACTAC	TTTTGCAACTTTAGCTGCGG	20	20	55.0	45.0	57.8	57.9	0.0	0.0	0.329035	66857	67255	485-491	1.000000	80.2
trnfM-CAU-atpI_p5	trnfM-CAU-atpI	5	TTATGAGCCTTGCGAGCTAC	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.357808	66857	67255	493-499	1.000000	79.9
rpoB-rpoA_p1	rpoB-rpoA	1	TTGCGGTCCTTCATAAGACG	GATTGCCTCCCAGAATCTCA	20	20	50.0	50.0	58.0	57.0	0.0	0.0	1.021618	84435	84684	703-703	0.500000	51.8
rpoB-rpoA_p2	rpoB-rpoA	2	AGTGTCAGCTTATCCATGCG	GATTGCCTCCCAGAATCTCA	20	20	50.0	50.0	58.1	57.0	0.0	0.0	1.071648	84435	84684	1024-1024	0.500000	51.4
rpoB-rpoA_p3	rpoB-rpoA	3	AAGACGAATTTCCGCTCGAA	GATTGCCTCCCAGAATCTCA	20	20	45.0	50.0	57.9	57.0	0.0	0.0	1.091582	84435	84684	689-689	0.500000	51.3
rpoB-rpoA_p4	rpoB-rpoA	4	ATTGACGGGTTAGTGTCAGC	GATTGCCTCCCAGAATCTCA	20	20	50.0	50.0	57.9	57.0	0.0	0.0	1.111797	84435	84684	1035-1035	0.500000	51.1
rpoB-rpoA_p5	rpoB-rpoA	5	TTCCTTAGACGACGATTGCG	GATTGCCTCCCAGAATCTCA	20	20	50.0	50.0	58.1	57.0	0.0	0.0	1.112891	84435	84684	718-718	0.500000	51.1
rpl36-rps8_p1	rpl36-rps8	1	GTTTATGCCTGGGGTTGGAA	AATGACAGATCGAGAGGCTC	20	20	50.0	50.0	58.1	56.8	0.0	6.3	1.244021	86443	87343	1065-1198	1.000000	73.9
rpl36-rps8_p2	rpl36-rps8	2	TTTATGCCTGGGGTTGGAAC	AATGACAGATCGAGAGGCTC	20	20	50.0	50.0	58.1	56.8	0.0	6.3	1.244021	86443	87343	1064-1197	1.000000	73.9
rpl36-rps8_p3	rpl36-rps8	3	CCTACGAATTAGGCGGCATT	AATGACAGATCGAGAGGCTC	20	20	50.0	50.0	58.1	56.8	13.1	6.3	1.296703	86443	87343	1022-1155	1.000000	73.6
rpl36-rps8_p4	rpl36-rps8	4	GTTTATGCCTGGGGTTGGAA	TCGACTAAAAGGAATTGGGGG	20	21	50.0	47.6	58.1	57.6	0.0	0.0	1.428060	86443	87343	1047-1180	1.000000	72.5
rpl36-rps8_p5	rpl36-rps8	5	TTTATGCCTGGGGTTGGAAC	TCGACTAAAAGGAATTGGGGG	20	21	50.0	47.6	58.1	57.6	0.0	0.0	1.428060	86443	87343	1046-1179	1.000000	72.5
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	TGGCAACTTTACGTAAGGCA	GACTTTCACCACGTCAAGGT	20	20	45.0	50.0	57.7	58.1	16.5	0.0	0.308225	103487	105060	1663-1667	1.000000	80.4
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	TGGCAACTTTACGTAAGGCA	TGACTTTCACCACGTCAAGG	20	20	45.0	50.0	57.7	58.1	16.5	0.0	0.309586	103487	105060	1664-1668	1.000000	80.4
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	ACGTAAGGCAGAGTTTGGTT	GACTTTCACCACGTCAAGGT	20	20	45.0	50.0	57.4	58.1	0.0	0.0	0.683521	103487	105060	1653-1657	1.000000	77.4
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	ACGTAAGGCAGAGTTTGGTT	TGACTTTCACCACGTCAAGG	20	20	45.0	50.0	57.4	58.1	0.0	0.0	0.684882	103487	105060	1654-1658	1.000000	77.4
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	TGGCAACTTTACGTAAGGCA	AGGTGACACTCTACCACTGA	20	20	45.0	50.0	57.7	57.3	16.5	5.4	0.935233	103487	105060	1647-1651	1.000000	75.4
rps16-rrn16_p1	rps16-rrn16	1	AATAAGCCGCTCCATGAAGG	TCATAGAGCGCCCTGTGATA	20	20	50.0	50.0	58.0	58.0	0.0	14.2	0.031752	109848	110097	1459-2129	1.000000	85.8
rps16-rrn16_p2	rps16-rrn16	2	AAGACAACCAGAGACGGGTA	TTGCACAAACAATGCTTGCA	20	20	50.0	40.0	58.0	58.0	0.0	0.0	0.025318	109848	110097	1174-1174	0.500000	59.8
rps16-rrn16_p3	rps16-rrn16	3	AATAAGCCGCTCCATGAAGG	ATCCAAAAACCGCCAGTTCT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.031752	109848	110097	1571-1571	0.500000	59.7
rps16-rrn16_p4	rps16-rrn16	4	AATAAGCCGCTCCATGAAGG	TTGCACAAACAATGCTTGCA	20	20	50.0	40.0	58.0	58.0	0.0	0.0	0.047076	109848	110097	1100-1100	0.500000	59.6
rps16-rrn16_p5	rps16-rrn16	5	GGCAGCGAATCGAACAAAAA	TCATAGAGCGCCCTGTGATA	20	20	45.0	50.0	58.0	58.0	6.9	14.2	0.049512	109848	110097	945-945	0.500000	59.6
trnN-GUU-rps15_p1	trnN-GUU-rps15	1	AAGACAACCAGAGACGGGTA	TTGCCTTACTCGCTACACAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.079917	125959	126078	2463-2904	1.000000	91.3
trnN-GUU-rps15_p2	trnN-GUU-rps15	2	AATAAGCCGCTCCATGAAGG	TTGCCTTACTCGCTACACAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.101675	125959	126078	2389-2830	1.000000	91.2
trnN-GUU-rps15_p3	trnN-GUU-rps15	3	GATTCTGACTCGATCGTCCG	TTGCCTTACTCGCTACACAC	20	20	55.0	50.0	58.0	57.9	15.9	0.0	0.100091	125959	126078	1629-1629	0.500000	59.2
trnN-GUU-rps15_p4	trnN-GUU-rps15	4	TCAGTGATTGGAGGTGGACT	TTGCCTTACTCGCTACACAC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.104574	125959	126078	2575-2575	0.500000	59.2
trnN-GUU-rps15_p5	trnN-GUU-rps15	5	TAAACAAGTAGGAGTGGCGC	GGAATAACACCGTCCTCCAC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.108897	125959	126078	1816-1816	0.500000	59.1
ndhI-ndhG_p1	ndhI-ndhG	1	ACCACTGTCAATAATTGGCGA	AAGACCCAATCCCAGGAAGA	21	20	42.9	50.0	57.9	57.9	0.0	0.0	1.126048	131978	133298	1462-1478	1.000000	73.4
ndhI-ndhG_p2	ndhI-ndhG	2	ACCACTGTCAATAATTGGCG	AAGACCCAATCCCAGGAAGA	20	20	45.0	50.0	56.4	57.9	0.0	0.0	1.649131	131978	133298	1462-1478	1.000000	69.2
ndhI-ndhG_p3	ndhI-ndhG	3	ACCACTGTCAATAATTGGCGA	ACCCAATCCCAGGAAGACTA	21	20	42.9	50.0	57.9	57.3	0.0	0.0	1.727467	131978	133298	1459-1475	1.000000	68.6
ndhI-ndhG_p4	ndhI-ndhG	4	ACCACTGTCAATAATTGGCGA	AGACCCAATCCCAGGAAGAC	21	20	42.9	55.0	57.9	58.7	0.0	0.0	1.776157	131978	133298	1461-1477	1.000000	68.2
ndhI-ndhG_p5	ndhI-ndhG	5	ACCACTGTCAATAATTGGCGA	GACCCAATCCCAGGAAGACT	21	20	42.9	55.0	57.9	58.7	0.0	0.0	1.776157	131978	133298	1460-1476	1.000000	68.2
rpl32-ndhF_p1	rpl32-ndhF	1	AGCGTGTCTACCGATTTCAC	AACAGGTCGTGTGAACCAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.124657	138570	139911	2584-2584	0.500000	59.0
rpl32-ndhF_p2	rpl32-ndhF	2	AGCGTGTCTACCGATTTCAC	ACAGGTCGTGTGAACCAAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.124657	138570	139911	2583-2583	0.500000	59.0
rpl32-ndhF_p3	rpl32-ndhF	3	CAGCGTGTCTACCGATTTCA	AACAGGTCGTGTGAACCAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.126028	138570	139911	2585-2585	0.500000	59.0
rpl32-ndhF_p4	rpl32-ndhF	4	CAGCGTGTCTACCGATTTCA	ACAGGTCGTGTGAACCAAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.126028	138570	139911	2584-2584	0.500000	59.0
rpl32-ndhF_p5	rpl32-ndhF	5	CGAAACACATTTCCCCAACG	ACAGGTCGTGTGAACCAAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.161715	138570	139911	1968-1968	0.500000	58.7
ndhG-ndhI_p1	ndhG-ndhI	1	AAGACCCAATCCCAGGAAGA	ACCACTGTCAATAATTGGCGA	20	21	50.0	42.9	57.9	57.9	0.0	0.0	1.126048	148181	149501	1462-1478	1.000000	73.4
ndhG-ndhI_p2	ndhG-ndhI	2	AAGACCCAATCCCAGGAAGA	ACCACTGTCAATAATTGGCG	20	20	50.0	45.0	57.9	56.4	0.0	0.0	1.649131	148181	149501	1462-1478	1.000000	69.2
ndhG-ndhI_p3	ndhG-ndhI	3	ACCCAATCCCAGGAAGACTA	ACCACTGTCAATAATTGGCGA	20	21	50.0	42.9	57.3	57.9	0.0	0.0	1.727467	148181	149501	1459-1475	1.000000	68.6
ndhG-ndhI_p4	ndhG-ndhI	4	AGACCCAATCCCAGGAAGAC	ACCACTGTCAATAATTGGCGA	20	21	55.0	42.9	58.7	57.9	0.0	0.0	1.776157	148181	149501	1461-1477	1.000000	68.2
ndhG-ndhI_p5	ndhG-ndhI	5	GACCCAATCCCAGGAAGACT	ACCACTGTCAATAATTGGCGA	20	21	55.0	42.9	58.7	57.9	0.0	0.0	1.776157	148181	149501	1460-1476	1.000000	68.2
rps15-trnN-GUU_p1	rps15-trnN-GUU	1	TTGCCTTACTCGCTACACAC	AAGACAACCAGAGACGGGTA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.079917	155442	155561	2463-2904	1.000000	89.1
rps15-trnN-GUU_p2	rps15-trnN-GUU	2	TTGCCTTACTCGCTACACAC	AATAAGCCGCTCCATGAAGG	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.101675	155442	155561	2389-2830	1.000000	89.1
rps15-trnN-GUU_p3	rps15-trnN-GUU	3	TTGCCTTACTCGCTACACAC	GATTCTGACTCGATCGTCCG	20	20	50.0	55.0	57.9	58.0	0.0	15.9	0.100091	155442	155561	1629-1629	0.500000	59.2
rps15-trnN-GUU_p4	rps15-trnN-GUU	4	TTGCCTTACTCGCTACACAC	TCAGTGATTGGAGGTGGACT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.104574	155442	155561	2575-2575	0.500000	59.2
rps15-trnN-GUU_p5	rps15-trnN-GUU	5	GGAATAACACCGTCCTCCAC	TAAACAAGTAGGAGTGGCGC	20	20	55.0	50.0	58.0	57.9	0.0	0.0	0.108897	155442	155561	1816-1816	0.500000	59.1
rrn16-rps16_p1	rrn16-rps16	1	GGAATAACACCGTCCTCCAC	TCGTCCGCTTGACAATACTG	20	20	55.0	50.0	58.0	58.0	0.0	4.4	0.029148	168947	169196	1898-1898	0.500000	59.8
rrn16-rps16_p2	rrn16-rps16	2	ACAAAGGAGAGCCTCTGTCT	TCGTCCGCTTGACAATACTG	20	20	50.0	50.0	58.0	58.0	0.0	4.4	0.031919	168947	169196	1929-1929	0.500000	59.7
rrn16-rps16_p3	rrn16-rps16	3	GGAATAACACCGTCCTCCAC	TCCACCGAATTCAACGATCC	20	20	55.0	50.0	58.0	58.0	0.0	6.2	0.038490	168947	169196	2431-2431	0.500000	59.7
rrn16-rps16_p4	rrn16-rps16	4	ACAAAGGAGAGCCTCTGTCT	TCCACCGAATTCAACGATCC	20	20	50.0	50.0	58.0	58.0	0.0	6.2	0.041260	168947	169196	2462-2462	0.500000	59.7
rrn16-rps16_p5	rrn16-rps16	5	CGACTCGACAAGACAACGAT	TCGTCCGCTTGACAATACTG	20	20	50.0	50.0	58.0	58.0	0.0	4.4	0.044117	168947	169196	2244-2244	0.500000	59.6