Hotspot

rps16

LSC · 4744-5864 · 1121 bp

Back to Agapanthus

Pi 0.00119
MarkerSeek score 59.70
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1121
variable_sites
2
pi
0.00119
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 GCAAGAAAAGAGCAAATTGTGG ACCGTTGAGCCAATGACTAT 40.9 45.0 57.3 56.9 1270-1272 bp 1.0 49.8
rps16_p2 GCAAGAAAAGAGCAAATTGTGG ATACCGTTGAGCCAATGACT 40.9 45.0 57.3 56.9 1272-1274 bp 1.0 49.8
rps16_p3 AGAAAAGAGCAAATTGTGGGT ACCGTTGAGCCAATGACTAT 38.1 45.0 56.2 56.9 1267-1269 bp 1.0 49.5
rps16_p4 AGAAAAGAGCAAATTGTGGGT ATACCGTTGAGCCAATGACT 38.1 45.0 56.2 56.9 1269-1271 bp 1.0 49.5
rps16_p5 GCAAGAAAAGAGCAAATTGTGG ACCGTTGAGCCAATGACTATT 40.9 42.9 57.3 57.6 1270-1272 bp 1.0 47.7