Hotspot

rpl16

LSC · 82584-83936 · 1353 bp

Back to Agapanthus

Pi 0.000985

MarkerSeek score 59.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1353
variable_sites: 2
pi: 0.000985
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TCATCCCCAATAACCCTTTTGT	ACAATTCTAATTCTATAGGGTTGA	40.9	29.2	57.6	53.2	1455-1456 bp	1.0	40.2
rpl16_p2	TCATCCCCAATAACCCTTTTGT	AACAATTCTAATTCTATAGGGTTGA	40.9	28.0	57.6	54.0	1456-1457 bp	1.0	40.2
rpl16_p3	TCATCCCCAATAACCCTTTTGT	ACAATTCTAATTCTATAGGGTTGAA	40.9	28.0	57.6	54.0	1455-1456 bp	1.0	40.2
rpl16_p4	TCATCCCCAATAACCCTTTTGT	AAACAATTCTAATTCTATAGGGTTGA	40.9	26.9	57.6	54.7	1457-1458 bp	1.0	40.2
rpl16_p5	TCATCCCCAATAACCCTTTTGT	AACAATTCTAATTCTATAGGGTTGAA	40.9	26.9	57.6	54.7	1456-1457 bp	1.0	40.2