Hotspot

rbcL

LSC · 56535-57977 · 1443 bp

Back to Agapanthus

Pi 0.00254

MarkerSeek score 60.30

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1443
variable_sites: 5
pi: 0.00254
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	AGACCTTGTTGTTGCGAGAA	AAAAGGATTGGGCCGAGTTT	45.0	45.0	57.7	58.0	1570 bp	1.0	77.6
rbcL_p2	AGACCTTGTTGTTGCGAGAA	AATTACTGAATTGTGCGCGC	45.0	45.0	57.7	58.1	1521 bp	1.0	76.8
rbcL_p3	AGACCTTGTTGTTGCGAGAA	TTAGGAAAAGGATTGGGCCG	45.0	50.0	57.7	57.9	1575 bp	1.0	76.5
rbcL_p4	AGACCTTGTTGTTGCGAGAA	TACTGAATTGTGCGCGCTTA	45.0	45.0	57.7	58.3	1518 bp	1.0	75.4
rbcL_p5	AGACCTTGTTGTTGCGAGAA	TGGGCCGAGTTTAATTGCAA	45.0	45.0	57.7	58.4	1562 bp	1.0	74.6