Hotspot

psaA

LSC · 40346-42598 · 2253 bp

Back to Agapanthus

Pi 0.000592

MarkerSeek score 57.60

Primer available yes

Label psaA

Feature Metadata

label_name: psaA
feature_type: gene
region: LSC
length_bp: 2253
variable_sites: 2
pi: 0.000592
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA_p1	GGGTCCTGAGCTAAACCTTG	GCGGGTCTCTTTGTATGTGT	55.0	50.0	57.9	57.9	2367 bp	1.0	78.4
psaA_p2	GGTCCTGAGCTAAACCTTGG	GCGGGTCTCTTTGTATGTGT	55.0	50.0	57.9	57.9	2366 bp	1.0	78.4
psaA_p3	GGGTCCTGAGCTAAACCTTG	TTGGCGGGTCTCTTTGTATG	55.0	50.0	57.9	57.9	2370 bp	1.0	78.3
psaA_p4	GGTCCTGAGCTAAACCTTGG	TTGGCGGGTCTCTTTGTATG	55.0	50.0	57.9	57.9	2369 bp	1.0	78.3
psaA_p5	GGTCCTGAGCTAAACCTTGG	GTTGGCGGGTCTCTTTGTAT	55.0	50.0	57.9	57.9	2370 bp	1.0	78.3