Hotspot

ndhA

SSC · 121897-123972 · 2076 bp

Back to Agapanthus

Pi 0.00128

MarkerSeek score 58.40

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2076
variable_sites: 4
pi: 0.00128
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ATGAACCCGGTCACCATAGG	TGGGAGAAGTTGATCGTTGA	55.0	45.0	59.2	56.5	2210 bp	1.0	58.7
ndhA_p2	CCGGTCACCATAGGGAACAT	TGGGAGAAGTTGATCGTTGA	55.0	45.0	59.2	56.5	2204 bp	1.0	58.7
ndhA_p3	ATTTATGAACCCGGTCACCA	TGGGAGAAGTTGATCGTTGA	45.0	45.0	56.8	56.5	2214 bp	1.0	58.6
ndhA_p4	TTTATGAACCCGGTCACCAT	TGGGAGAAGTTGATCGTTGA	45.0	45.0	56.8	56.5	2213 bp	1.0	58.6
ndhA_p5	CCGGTCACCATAGGGAACAT	TGGGAGAAGTTGATCGTTGAA	55.0	42.9	59.2	57.2	2204 bp	1.0	56.7