Hotspot

atpF

LSC · 12455-13836 · 1382 bp

Back to Agapanthus

Pi 0.000965

MarkerSeek score 59.40

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1382
variable_sites: 2
pi: 0.000965
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	ACTAATTTCGTCAGCTCGAA	GGCGAGCCAAGTGATACAAA	40.0	50.0	54.9	58.6	1551-1552 bp	1.0	50.5
atpF_p2	ACTAATTTCGTCAGCTCGAA	GGCGAGCCAAGTGATACAA	40.0	52.6	54.9	57.8	1551-1552 bp	1.0	45.7
atpF_p3	ACTAATTTCGTCAGCTCGAA	GCGAGCCAAGTGATACAAAA	40.0	45.0	54.9	56.7	1550-1551 bp	1.0	44.7
atpF_p4	ACTAATTTCGTCAGCTCGAA	AAAGGGCGAGCCAAGTGATA	40.0	50.0	54.9	59.4	1555-1556 bp	1.0	43.9
atpF_p5	TACTAATTTCGTCAGCTCGA	GGCGAGCCAAGTGATACAAA	40.0	50.0	54.0	58.6	1552-1553 bp	1.0	43.6