Hotspot

ndhF-rpl32

SSC · 114282-115189 · 908 bp

Back to Agapanthus

Pi 0.00294
MarkerSeek score 60.60
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
908
variable_sites
4
pi
0.00294
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGTTGCTGTCGGAATAAGCA ACTGACTTTGCTAAAGAAAAAGCT 45.0 33.3 57.8 57.5 1120 bp 1.0 43.2
ndhF-rpl32_p2 TGTTGCTGTCGGAATAAGCA TGCAGCTAAATATCCCTTTTTCT 45.0 34.8 57.8 56.2 1091 bp 1.0 40.6
ndhF-rpl32_p3 TGTTGCTGTCGGAATAAGCA ACTGACTTTGCTAAAGAAAAAGC 45.0 34.8 57.8 56.2 1120 bp 1.0 40.5
ndhF-rpl32_p4 TGTTGCTGTCGGAATAAGCA AGCTTTTATTGCAGCTAAATATCCC 45.0 36.0 57.8 58.0 1100 bp 1.0 40.3
ndhF-rpl32_p5 TGTTGCTGTCGGAATAAGCA GCTTTTATTGCAGCTAAATATCCCT 45.0 36.0 57.8 58.0 1099 bp 1.0 40.3