Hotspot

ndhC-trnV-UAC

LSC · 51275-52818 · 1544 bp

Back to Agapanthus

Pi 0.000864

MarkerSeek score 59.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1544
variable_sites: 2
pi: 0.000864
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGCCAAGATAGGAATCGCAC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.0	57.8	1646-1649 bp	1.0	78.3
ndhC-trnV-UAC_p2	AATCGGTGCTAAAACTCCGG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.3	57.8	1676-1679 bp	1.0	76.5
ndhC-trnV-UAC_p3	TGCCAAGATAGGAATCGCAC	AGCCCAATGCAACAAAATGC	50.0	45.0	58.0	58.5	1728-1731 bp	1.0	76.1
ndhC-trnV-UAC_p4	GGTCCTTCACTAATCGGTGC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.3	57.8	1687-1690 bp	1.0	75.9
ndhC-trnV-UAC_p5	TGCCAAGATAGGAATCGCAC	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.0	57.5	1645-1648 bp	1.0	75.5