Hotspot

psbM-trnD-GUC

LSC · 30409-31473 · 1065 bp

Back to Agapanthus

Pi 0.00188

MarkerSeek score 60.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1065
variable_sites: 3
pi: 0.00188
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	CCTCGCTGGGATTGTAGTTC	45.0	55.0	55.8	58.1	1190-1193 bp	1.0	62.1
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	AGTTCAATCGGTCAGAGCAC	45.0	50.0	55.8	57.9	1175-1178 bp	1.0	61.9
psbM-trnD-GUC_p3	AGAATGAACAGTGCAGTAGC	TACACCTCGCTGGGATTGTA	45.0	50.0	55.8	58.1	1194-1197 bp	1.0	61.4
psbM-trnD-GUC_p4	AGTGCGAGAATATTGACTTCCA	CCTCGCTGGGATTGTAGTTC	40.9	55.0	57.5	58.1	1166-1169 bp	1.0	60.2
psbM-trnD-GUC_p5	AGTGCGAGAATATTGACTTCCA	AGTTCAATCGGTCAGAGCAC	40.9	50.0	57.5	57.9	1151-1154 bp	1.0	60.0