Hotspot

psbK-psbI

LSC · 7940-8366 · 427 bp

Back to Agapanthus

Pi 0.00312

MarkerSeek score 56.50

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 427
variable_sites: 2
pi: 0.00312
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	CTTTGTTTGGCAAGCTGCTA	GGCCCGGATCGTTAGATAAG	45.0	55.0	57.2	57.6	549 bp	1.0	71.0
psbK-psbI_p2	CTTTGTTTGGCAAGCTGCTAT	GGCCCGGATCGTTAGATAAG	42.9	55.0	57.7	57.6	549 bp	1.0	66.9
psbK-psbI_p3	CTTTGTTTGGCAAGCTGCTA	TCCTCACGTCCAGGATTACG	45.0	55.0	57.2	58.9	568 bp	1.0	66.7
psbK-psbI_p4	TGCCTTTTTCAGTCCAATCG	GGCCCGGATCGTTAGATAAG	45.0	55.0	56.6	57.6	612 bp	1.0	66.4
psbK-psbI_p5	GCCTTTTTCAGTCCAATCGT	GGCCCGGATCGTTAGATAAG	45.0	55.0	56.6	57.6	611 bp	1.0	66.3