Hotspot

rps16-trnQ-UUG

LSC · 5865-7343 · 1479 bp

Back to Agapanthus

Pi 0.000902

MarkerSeek score 59.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1479
variable_sites: 2
pi: 0.000902
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1545-1552 bp	1.0	78.7
rps16-trnQ-UUG_p2	AAGTCGCACGTTGCTTTCTA	CTCGGAGGTTCGAATCCTTC	45.0	55.0	58.1	57.8	1549-1556 bp	1.0	77.7
rps16-trnQ-UUG_p3	AGCGAATCGTGTCCTTCAAG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.3	58.1	1562-1569 bp	1.0	77.6
rps16-trnQ-UUG_p4	AGAATCCACAGCGAATCGTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	1571-1578 bp	1.0	77.0
rps16-trnQ-UUG_p5	AAGAATCCACAGCGAATCGT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.6	58.1	1572-1579 bp	1.0	76.8