Hotspot

trnK-UUU-rps16

LSC · 4046-4743 · 698 bp

Back to Agapanthus

Pi 0.00287

MarkerSeek score 52.90

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 698
variable_sites: 3
pi: 0.00287
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	799-800 bp	1.0	77.4
trnK-UUU-rps16_p2	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.2	58.2	798-799 bp	1.0	77.4
trnK-UUU-rps16_p3	TCTACCAATAGCCTGGACGA	GGTGCTCAACCTACAGGAAC	50.0	55.0	57.6	58.2	864-865 bp	1.0	75.2
trnK-UUU-rps16_p4	TACCGTTGAGTTAGCAACCC	GGTGCTCAACCTACAGGAAC	50.0	55.0	57.5	58.2	785-786 bp	1.0	75.1
trnK-UUU-rps16_p5	AGTCGCACTTAAAAGCCGAG	GGTGCTCAACCTACAGGAAC	50.0	55.0	58.6	58.2	810-811 bp	1.0	74.2