Hotspot

rrn16S

IRa · 141890-143381 · 1492 bp

Back to Afzelia

Pi 0.00697

MarkerSeek score 39.60

Primer available yes

Label rrn16S

Feature Metadata

label_name: rrn16S
feature_type: rRNA
region: IRa
length_bp: 1492
variable_sites: 26
pi: 0.00697
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16S_p1	CAGACGTAGCTCGCTTCTTT	TGTCTACGAACAAGGAAGCT	50.0	45.0	58.0	56.5	1579-1607 bp	1.0	70.6
rrn16S_p2	TGTGAAGCAGTGTCAAACCA	TGTCTACGAACAAGGAAGCT	45.0	45.0	57.9	56.5	1554-1582 bp	1.0	69.8
rrn16S_p3	TTCTTTTGGGGTGTGAAGCA	TGTCTACGAACAAGGAAGCT	45.0	45.0	57.8	56.5	1565-1593 bp	1.0	69.6
rrn16S_p4	CAGACGTAGCTCGCTTCTTT	TTGTCTACGAACAAGGAAGCT	50.0	42.9	58.0	57.3	1580-1608 bp	1.0	68.6
rrn16S_p5	TCTTTTGGGGTGTGAAGCAG	TGTCTACGAACAAGGAAGCT	50.0	45.0	58.3	56.5	1564-1592 bp	1.0	68.3